JIST

Abstract

'FINIT' comes under a broad spectrum of common household insecticide which chemically comprises of Malathion and Pyrethrins. Commonly it is used in prevention of insects such as flies, mosquitos, moths, cockroaches and ants etc. Its unsupervised use leads to accidental poisoning along with the intentional suicidal poisoning and hence its analysis is very important for medico legal purposes. Routinely, High Performance Liquid Chromatography, Gas Chroma­tography, Gas Chromatography-Mass-Spectroscopy and Liquid Chromatography-Mass Spectroscopy are used for analysis of Malathion & Pyrethrins. These techniques are not only costly but also require more sophisticated instruments. An attempt has been made to develop a new method for analysis of 'FINIT' in biological samples namely blood and urine using the different solvent system as mobile phase of Thin Layer Chromatography {TLC). 'FINIT' was extracted from blood and urine sample using liquid-liquid extraction method and analyzed by TLC. Developed plates were viewed under UV light followed by spray of chromogenic reagents which successfully increased the sensitivity without dispensing with the simplicity of the method. The method developed is a simple, rapid, inexpensive, non-destructive and reproducible which can be performed in any laboratory easily.