JIST

Abstract

Over the years, Forensic Science Laboratories receives cases of accidental as well as intentional bromadiolone intoxication. Bromadiolone is an anticoagulant   inhalant rodenticide used in rural and urban areas to control rodent. Over the last many decades, it has been observed that the use of rodenticide has been increased in promoting public health in hospitals, outdoor and home, with many generations coming into existence. The easy availability of rodenticide for use in public hygiene and agricultural has caused them to be used for suicidal and homicidal purposes. Bromadiolone, one of the most commonly available rat kill poison acts by inhibiting the synthesis of Vitamin K in blood, thereby causing internal bleeding and hemorrhage. Reported literature on the detection of bromadiolone poisoning mention detection of TLC spot by visualization under UV light only. The present study aimed for developing spray reagent for visualization of the Bromadiolone spots on Thin layer chromatography plates.